Plasmid

Part:BBa_K4190012:Design

Designed by: N/A   Group: iGEM22_UCSC   (2022-09-21)


mg-Int-leu2-kan_1420 Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5007
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 776
    Illegal PstI site found at 785
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5007
    Illegal SpeI site found at 776
    Illegal PstI site found at 785
    Illegal NotI site found at 2708
    Illegal NotI site found at 4471
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5007
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 776
    Illegal PstI site found at 785
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5007
    Illegal XbaI site found at 5016
    Illegal SpeI site found at 776
    Illegal PstI site found at 785
    Illegal NgoMIV site found at 701
    Illegal NgoMIV site found at 4801
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 590
    Illegal BsaI.rc site found at 5181
    Illegal SapI.rc site found at 5365


Source

Rohinton Kamakaka at UCSC.